Efficacy of quinolones and cephalosporins against antibiogram of Escherichia coli isolated from chickens

Authors

  • Ranjhan Ali Lakho Department of Ve terinary Microbiology, Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University, Tandojam 70060, Pakistan
  • Shahid Hussain Abro Department of Ve terinary Microbiology, Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University, Tandojam 70060, Pakistan
  • Muhammad Tarique Tunio Department of Agricultural Sciences, Faculty of Sciences, Allama Iqbal Open University, Islamabad
  • Mohsina Zubair Department of Chemistry, Preston University, Islamabad
  • Rani Abro Department of Animal Nutrition, Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University, Tandojam 70060, Pakistan
  • Rahmatullah Rind Department of Ve terinary Microbiology, Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University, Tandojam 70060, Pakistan
  • Riaz Ahmed Leghari Department of Ve terinary Microbiology, Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University, Tandojam 70060, Pakistan
  • Kanwar Kumar Malhi Department of Ve terinary Microbiology, Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University, Tandojam 70060, Pakistan
  • Muhammad Rafique Rind Department of Biotechnology, Sindh Agriculture University, Tandojam 70060, Pakistan
  • Asghar Ali Kamboh Department of Ve terinary Microbiology, Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University, Tandojam 70060, Pakistan

Keywords:

Antibiogram, Cephalosporins, Chicken, E. coli, Quinolones

Abstract

Colibacillosis is an acute septicemia disease caused by E. coli producing considerable morbidity
and mortality especially in poultry. Quinolones and cephalosporins have been used in treatment of various
infections. Therefore, this study was designed to evaluate the efficacy of quinolones and cephalosporins
against the antibiogram of E. coli isolated from chicken flocks. The 100 blood samples from liver and intestine
were collected from different poultry vendors surrounding of Tandojam and Hyderabad, Sindh, Pakistan. The
isolated organism was cultured on nutrient and blood agar media. The cultural, morphological and
biochemical characteristics were observed for the confirmation of the isolated organism. The minimum
inhibitory concentration (MIC) of different antibiotics against E. coli was performed by serially diluting
antibiotics ciprofloxacin, metronidazole, cefipime as 0.4 µg/ml, 0.8 µg/ml, 1.6 µg/ml, 3.2 µg/ml and 6.4 µg/ml,
12.5 µg/ml, 25 µg/ml and 50 µg/ml, respectively. The mean zones of inhibition of ciprofloxacin, ofloxacin,
enrofloxacin, norfloxacin, cefepime, ceftazidimine and cefoxitin against the antibiogram of E. coli were
recorded as 14, 11, 12, 11, 11, 11, and 11 mm, respectively. MIC results indicated that ciprofloxacin was found
to be more effective to inhibit the growth of E. coli. It was found that antibiotics of quinolones group namely
ciprofloxacin, enroflaxcin and ofloxacin as well as cephalosporin group namely cefipime, ceftazidime and
cefoxitin were found to be effective to isolate E. coli.

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Published

2023-06-05

How to Cite

Lakho, R. A., Abro, S. H., Tunio, M. T., Zubair, M., Abro, R., Rind, R., Leghari, R. A., Malhi, K. K., Rind, M. R., & Kamboh, A. A. (2023). Efficacy of quinolones and cephalosporins against antibiogram of Escherichia coli isolated from chickens. Journal of Pure and Applied Agriculture, 2(1). Retrieved from https://ojs.aiou.edu.pk/index.php/jpaa/article/view/1144