Isolation and identification of Agrobacterium tumefaciens from the galls of peach tree

Abstract

Peach (Prunus perisca) is a very important fruit and it is attractive all over the world due to its delicious aroma and flavor. Peach is susceptible to various types of pathogens that result in decline of its fruit production. An important pathogen, Agrobacterium tumefaciens attacks on peach plant and it causes crown gall disease. The present study was conducted to identify the peach plants infected with Agrobacterium tumefaciens and isolate as well as identify the bacterium from the crown gall samples. These samples were collected from different locations including district Swat and Shangla. The bacterium was isolated from the
samples using MacConkey selection media that is used specifically for identification of Agrobacterium
tumefaciens.