Efficacy of quinolones and cephalosporins against antibiogram of Escherichia coli isolated from chickens

Abstract

Colibacillosis is an acute septicemia disease caused by E. coli producing considerable morbidity
and mortality especially in poultry. Quinolones and cephalosporins have been used in treatment of various
infections. Therefore, this study was designed to evaluate the efficacy of quinolones and cephalosporins
against the antibiogram of E. coli isolated from chicken flocks. The 100 blood samples from liver and intestine
were collected from different poultry vendors surrounding of Tandojam and Hyderabad, Sindh, Pakistan. The
isolated organism was cultured on nutrient and blood agar media. The cultural, morphological and
biochemical characteristics were observed for the confirmation of the isolated organism. The minimum
inhibitory concentration (MIC) of different antibiotics against E. coli was performed by serially diluting
antibiotics ciprofloxacin, metronidazole, cefipime as 0.4 μg/ml, 0.8 μg/ml, 1.6 μg/ml, 3.2 μg/ml and 6.4 μg/ml,
12.5 μg/ml, 25 μg/ml and 50 μg/ml, respectively. The mean zones of inhibition of ciprofloxacin, ofloxacin,
enrofloxacin, norfloxacin, cefepime, ceftazidimine and cefoxitin against the antibiogram of E. coli were
recorded as 14, 11, 12, 11, 11, 11, and 11 mm, respectively. MIC results indicated that ciprofloxacin was found
to be more effective to inhibit the growth of E. coli. It was found that antibiotics of quinolones group namely
ciprofloxacin, enroflaxcin and ofloxacin as well as cephalosporin group namely cefipime, ceftazidime and
cefoxitin were found to be effective to isolate E. coli.