Investigation of interactions of acetzolamide with tricil at pH = 3.0, pH = 7.0 and pH = 9.5 through UV-Vis spectroscopy and cyclic voltammetry

Abstract

The interaction of antihypertensive drug acetazolamide (Azm) with antacid drug tricil was investigated at pH = 3.0, pH = 7.0 and pH = 9.5 with the objective of monitoring their interactive pharmacokinetics during digestion and absorption in human body. The variation in absorbance and current of one drug caused by the addition of another drug revealed that tricil interacted with Azm in both acidic (pH = 3.0) and basic conditions (pH = 9.5). But these twodrugs did not interact with each other at neutral pH (pH = 7.0). Further, in acidic conditions Azm-tricil formed 1:1 complex while these formed 1:2 complex at pH = 9.5 (basic conditions).